Pengaruh Lama Pencucian Terhadap Kadar Vitamin B1 Pada Beras Putih Dan Beras Merah Secara Spektrofotometer Visibel

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A research about analysis of vitamin B₁ on the effeccts of long leaching on levels of vitamin B₁ on white rice and red rice using UV-Visible spectrophotometry. Vitamin B₁ reacted with bromthymol blue for compose ion-association complex. For improve solubility of Vitamin B₁ complex compounds with bromthymol blue be added polivinyl alcohol as a solubilization substance, and added amonia buffer as pH buffer, that measured with wavelength of maximum absorbance was 431,0 nm. Average (%) Vitamin B₁ level of white rice after leaching minutes 1 is 0,0166. Average vitamin B₁ level of red rice after leaching minutes 1 is 0,0262. The highest level of vitamin B₁ from the white rice and red rice was found on minute 1 after leaching. From the statistical analysis one way ANOVA using SPSS 17 program found a significant difference (p<0,05) from each sample.