Produksi Bioplastik Poli (3-Hidroksibutirat) (P(3HB)) Secara Proses Fermentasi Menggunakan Bakteri Bacillus brevis FAAC-20801 Dari Minyak Kelapa Sawit Sebagai Sumber Karbon

Krisyanella Krisyanella; Mimi Susanti; Akmal Djamaan

doi:10.52689/higea.v2i1.20

Produksi Bioplastik Poli (3-Hidroksibutirat) (P(3HB)) Secara Proses Fermentasi Menggunakan Bakteri Bacillus brevis FAAC-20801 Dari Minyak Kelapa Sawit Sebagai Sumber Karbon

Krisyanella Krisyanella, Mimi Susanti, Akmal Djamaan

Sari

The production of bioplastic P(3HB) with fermentation process by Bacillus brevis FAAC-20801 from palm oil as carbon has been carried out. The fermentation process was conducted throug at pH 7, 37oC and agitation rate of 200 rpm for 60 hours. The characterization of the bioplastic production was based on cell growth, P(3HB) content and P(3HB) percentage. Results showed that the optimum time was 48 hours, with fermentation biomassa 277 mg, P(3HB) content of 7.2 mg/ 20 mg biomassa (36 % b/b). Fermentation process in bioreactor produced P(3HB) higher than in rotary shaker incubator.

Kata Kunci

Bacillus brevis; Bioplastic (P3HB); Fermentation Process

Teks Lengkap:

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Referensi

Anderson. A. J., Dawes E.A., 1990, Occurance, Metabolism, Metabolic Role, and Industrial Uses of Bacterial Polyhydroxyalkanoates, Microbial. Rev, 54.450-472.

Djamaan. A., 2000, Penghasilan Biopolimer oleh mikroorganisme, Farmasi FMIPA, Universitas Andalas, Padang.

Doi. Y., 1990, Microbial Polyester, VCH Publisher. Inc., New York.

Ketaren, S., 1986, Minyak dan Lemak Pangan, Universitas Indonesia , Jakarta.

Lee. S. Y., 1996, Bacterial Polyhydroxy alkanoates, Biotechnol Bioeng. 49 : 1-14.

Lee. S. Y., 1996, Plastic Bacteri, Progress and Project for Polyhydroxyalkanoat Production in Bacteria, 14 : 1-7.

Majid. M.I.A., K. Hori., M.Akiyama Y.Doi., 1994, Production of Poly (3-hydroxybutyrate) from Palm Oil By Alcaligenes Sp In Biodegradable Plastic and Polymer., Amsterdam.

Pelczar. M.J., 1986, Dasar-Dasar Mikrobiologi, Universitas Indonesia,Jakarta.

Wardhana, W. Arya., 1994, Dampak Pencemaran Lingkungan, Andi, Yogyakarta.

DOI: http://dx.doi.org/10.52689/higea.v2i1.20